Identification and confirmation of new molecular markers linked to the fertility restorer genes in sugar beet
Identification and confirmation of new molecular markers linked to the fertility restorer genes in sugar beet / Peyman Norouzi, Abazar Rajabi
p. 335-341 ; 29cm
수록자료: Journal of crop science and biotechnology. Korean Society of Crop Science. vol.21 no.4(2018 December), p. 335-341 21:4<335 ISSN 1975-9479↔ 저자: Peyman Norouzi, Associate professor of Sugar Best Seed Institute, Agricultural Research, Education and Extension Organization (AREEO) 저자: Abazar Rajabi, Associate professor of Sugar Best Seed Institute, Agricultural Research, Education and Extension Organization (AREEO)
It is of special importance to track the fertility restoring alleles in plants such as sugar beet in which cytoplasmic male sterility (CMS) is used to produce hybrid seed, so that the possibility of detection of presence and absence of specific restoring alleles facilitates to a great extent the development of new O-type lines. For tagging of fertility restorer genes in sugar beet, an F₂ population was used. At first, fertility scoring of F₂ single plants in the field was done and the population was divided to four groups including fertile, half fertile, yellow sterile, and white sterile. Then, leaf sampling from the F₂ single plants was accomplished for DNA extraction. Polymorphism of random primers of RAPD and STS primers was investigated in fertile and sterile bulks by bulk segregant analysis method. Several RAPD and STS primers produced polymorphism in fertile and sterile bulks. After testing the selected primers on the DNA of single plants of the bulk, F₂ population and other sugar beet genotypes, one STS repulsion marker and one coupling marker were identified to distances of 17 and 8% recombination frequencies, respectively. In this research, the molecular basis of the genomic regions of fertility restoring loci of sugar beet was studied for the first time in Iran. These markers could be used for preliminary selection of O-type plants from pollinator populations and hence the time and costs related to pair crosses for identification of O-type character could be saved.